Expression of vascular endothelial growth factor receptors in tumor and stromal cells of tongue squamous cell carcinoma
박봉욱, 변준호, 하영술, 김덕룡, 정인교, 김종렬, 김욱규, 박봉수, 김규천,
소속 상세정보
박봉욱 ( Park Bong-Wook ) - 경상대학교병원 구강악안면외과학교실
변준호 ( Byun June-Ho ) - 경상대학교병원 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 소속확인중
김덕룡 ( Kim Deok-Ryong ) - 소속확인중
정인교 ( Jung In-Kyo ) - 부산대학교 치과대학 구강안면외과학교실
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치과대학 구강안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치과대학 구강안면외과학교실
박봉수 ( Park Bong-Soo ) - 부산대학교 치과대학 구강해부학교실 및 구강생물공학연구소
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치과대학 구강해부학교실 및 구강생물공학연구소
KMID : 0355620070330010011
Abstract
This study was to evaluate the expression of vascular endothelial growth factor receptors (VEGFRs) in tumor and stromal cells of tougue squamous cell carcinoma (SCC). We also wanted to characterize the differences, from the angiogenic aspect, between cancer-associated stromal cells and non-malignant stromal cells. Paraffin-embedded tumor specimens from eleven patients with tongue SCCs were studied. Immunohistochemical staining for VEGFR-1,-2, and -3 was performed on the tumor cells, stromal fibroblasts and tumor-associated macrophages of the specimens. The expression of all 3 receptors was detected in the tumor cells themselves of the biopsy specimens. All 3 receptors were also expressed on stromal cells, except that VEGFR-2 was not expressed in stromal fibroblasts. In radical excision specimens, the staining intensity for VEGFR-1, -2 in the tumor cells and VEGFR-1,-3 in the tumor-associated macrophages was significantly lower than that in the biopsy specimens (P < 0.05). By using the general marker of fibroblast and macrophage, 5B5 and CD68, respectively, we performed double immunofluorescence staining for 5B5 and each VEGFR in the stromal fibroblasts and for CD68 and each VEGFR in the tumor-associated macrophages of the radical excision specimens. We used 4 cases of fibroma and 4 cases of chronic inflammation tissue as the controls. It was found that only each marker was expressed in the control group, however, 5B5/VEGFR-1 and 5B5/VEGFR-3 in the stromal fibroblasts, and CD68/VEGFR-1 and CD68/VEGFR-3 in the tumor-associated macrophages were double stained in the radical excision specimens. Although our study used small number of specimens, the results of our study showed that in tongue SCC, in association with the angiogenesis, the stromal cells showed the activated phenotype and this was different from the nonmalignant stromal cells.
키워드
Vascular endothelial growth factor receptor (VEGFR);Stromal fibroblast;Tumor-associated macrophage
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